Fig. 1.

Hsp21 chaperone activity towards heat-treated MDH. The model substrate MDH was heat-treated by incubation at 45 °C in absence or presence of varying amounts of Hsp21. Thermal-induced precipitation of MDH was followed as light-scattering increase over time, and the suppression of light-scattering increase in presence of Hsp21 used to assess the chaperone activity. Samples assayed in duplicate or triplicate are marked with same color. The numbers of the ratios in the legend refer to concentrations (Hsp21/MDH) expressed in micromolars. a Hsp21/MSH molar ratios 1/1 and above, under different salt conditions (*): 1, low salt; 2, 0.1 M NaCl; 3, 0.2 M Na₂SO₄. b Hsp21/MDH molar ratios 1/1 and below, in 0.1 M NaCl