Table 1.
Crosslinks identified between Hsp21 and MDH
| Theoretical (MH)+ (Da) | Matched Hsp21 sequence | Matched MDH sequence | Hsp21 res. | MDH res. | Observations | Ox. Met observationa | Fig. S-2b |
|---|---|---|---|---|---|---|---|
| 1503.7412 | M1QDQR5 | K81PGMTR86 | 1 | 81 | 3 | 2 | A-C |
| 1870.9234 | M1QDQR5 | K133HGVYNPNK141 | 1 | 133 | 5 | 1 | D, E |
| 2087.0807 | M1QDQR5 | I206QEAGTEVVKAK217 | 1 | 215 | 7 | 1 | F, G |
| 1388.7208 | M1QDQR5 | K273GIEK277 | 1 | 273 | 2 | 1 | H, I |
| 1842.9747 | M1QDQR5 | G274IEKNLGIGK283 | 1 | 277 | 11 | J | |
| 1360.7258 | M1QDQR5 | A301SIKK305 | 1 | 304 | 2 | 1 | K, L |
| 1650.8161 | M1QDQR5 | K305GEEFVK311 | 1 | 305 | 2 | M | |
| 1926.0157 | G19NQGSSVEKRPQQR32 | A1K2 | 27 | 1 | 3 | N | |
| 2544.3170 | G19NQGSSVEKRPQQR32 | K305GEEFVK311 | 27 | 305 | 3 | O | |
| 1605.8852 | T172KVER176 | K305GEEFVK311 | 173 | 305 | 4 | P |
aThe observation(s) of the same peptide pair but with one or two methionine residues oxidized strengthens the identification, and in this case, the MS spectrum of the oxidized version of the crosslink is also shown in Fig. S-2 (Supplementary information). This type of observation was only counted once, even if it was observed in different data sets
bThe capital letter refers to where the MS spectrum is shown in Fig. S-2 (Supplementary information). MS/MS spectra of precursor masses 2087.0807, 1842.9747, and 1360.7258 Da can be found in Fig. S-3 (Supplementary information)