Figure 2.

A, Origin of RFD constructs used in this study. Three constructs (pMAJ1, pMAJ2, and pMAJ3) were prepared in the binary vector pROK2 from a cDNA corresponding to an A. majus DFR gene. The 5′→3′ of the original DFR sequence is indicated by ⇐. B, Schematic representation of T-DNA of control and antisense gene constructs. RB, Right border of T-DNA; LB, left border of T-DNA. Positions of nptII gene and AS-DFR constructions within T-DNA are indicated. nptII, CaMV, and nos primer positions are marked with > and <. Sizes of predicted PCR products from the control construct and from the three AS-DFR constructs are indicated.