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RT-PCR analysis of cDNA prepared from selected S33 control and pMAJ2 lines. Amplifications from selected lines were performed as outlined in Methods. The Plasmid lane is the amplification product from 20 pg of pJAM212 (A. majus DFR cDNA). The arrow indicates the predicted size of the amplification product derived from pMAJ2. DNA-marker lane is 2.5 μg of a 100-bp ladder (Pharmacia-Biotech).
