Table II.

Control and RFD lines in the S33 genotype analyzed in this study and a summary of PCR analysis

Description of Line	Designation	nptII	CaMV-nos
Recipient (nontransformed control)	S33	−	−
A. rhizogenes transformed	C26, C28, C32	−	−
Cotransformed with pROK2 (vector control)	C33/3, C33/5, C33/10	+	+
Cotransformed with pMAJ2	RFD4, RFD7, RFD8, RFD19, RFD38, RFD49, RFD50	+	+

Details of control and antisense constructs (pROK2, pMAJ1, pMAJ2, and pMAJ3) and derived amplification products are shown in Figure 2. Amplification products with nptII primers confirm the transfer of the selectable marker from binary vector T-DNA to L. corniculatus genomic DNA. CaMV-nos amplification products confirm the presence of promoter and terminator sequences and produce characteristically sized products from each of the four constructs in this study. +, Product noted; −, no product after amplification.