Table V.
PCR analysis of progeny from an effective RFD (RFD8)
| Line | nptII | CaMV-nos |
|---|---|---|
| RFD8 (five copies of transgene) | + | + |
| S50 | − | − |
| 7 | + | + |
| 13 | + | − |
| 15 | + | + |
| 18 | + | − |
| 22 | + | + |
| 23 | + | + |
| Remainder | − | − |
| Transgene inheritance | 25% | 17% |
Parental lines were analyzed together with 24 progeny lines (1–24). Amplification products with nptII primers confirm the presence of the selectable marker from binary vector T-DNA in genomic DNA of parental and progeny lines. CaMV-nos amplification products confirm the presence of promoter and terminator sequences and the antisense DFR fragment from pMAJ2. +, Product noted; −, no product noted after amplification.