Table VI.
PCR analysis of progeny from an ineffective RFD (RFD19)
| Line | nptII | CaMV-nos |
|---|---|---|
| RFD19 (three copies of transgene) | + | − |
| S50 | − | − |
| 1 | + | + |
| 2 | + | + |
| 4 | + | + |
| 9 | + | + (−) |
| 11 | + | − |
| 12 | + | + |
| 13 | + | + |
| 14 | + | + |
| 20 | + | + (−) |
| 22 | + | − |
| Remainder | − | − |
| Transgene inheritance | 48% | 38 (29)% |
Parental lines were analyzed together with 21 progeny lines (1–22). Amplification products with nptII primers confirm the presence of the selectable marker from binary vector T-DNA in genomic DNA of parental and progeny lines. CaMV-nos amplification products confirm the presence of promoter and terminator sequences and the antisense DFR fragment from pMAJ2. +, Product noted; −, no product noted; +(−), product not consistently observed after amplification.