Table 1.
PTC containing genes selected for RT-PCR and Sanger sequencing of cDNA. All four were Sanger sequenced to confirm that the PTC mutation observed was not a sequencing error in the Illumina data. Sanger sequencing of RT-PCR products showed that each of these genes are transcribed despite the presence of a PTC mutation.
|
D. pseudoobscura ID |
D mel Ortholog |
Genomes with PTC mutation |
Predicted % truncation |
Prominent GO notes for D. mel ortholog |
|---|---|---|---|---|
| GA15892 | SNF1A | 2 – Flagstaff14, Mather32 | 51.87 | establishment or maintenance of epithelial cell apical/basal polarity |
| GA19967 | Mth | 14 – Found in all species except D. miranda |
89.86 | determination of adult lifespan |
| GA21687 | Cht5 | 1 - Flagstaff14 | 96.61 | chitin catabolic process |
| GA22859 | Cyp4d2 | 1 – Pikes Peak 1134 | 87.05 | oxidation-reduction process |