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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2012 Oct 23;265(2):181–189. doi: 10.1016/j.taap.2012.10.009

Figure 4. Prenatal Cd exposure altered the relative percentage of a population of CD4+ natural T regulatory cells (nTreg) in the spleens of 20 week-old offspring.

Figure 4

Single cell suspensions were prepared for flow cytometry and stained with anti-CD4, anti-CD25 and anti-FoxP3 to quantify nTreg cells as described in the Materials and Methods. Each bar represents the mean ± SEM. Data are representative of 2 experiments where the n equals at least 5 in each group. *p<0.05