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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Cell Signal. 2012 Sep 7;25(1):247–254. doi: 10.1016/j.cellsig.2012.09.003

Figure 3. USP4 deubiquitinates Lys63-linked TAK1 polyubiquitination and inhibits Dox-induced NF-κB activation.

Figure 3

(A) Expression vectors encoding HA-Ub-K63 and FLAG-TAK1 were co-transfected into HEK-293T cells with control vector or expression vectors encoding MYC-USP4. Thirty six hours after transfection, cells were treated with Dox at the indicated time points or left untreated. FLAG-TAK1 proteins in the transfected cells were immunoprecipitated with anti-FLAG antibodies and immunoblotted with anti-HA antibodies to detect the presence of polyubiquitinated FLAG-TAK1. (B) USP4 stable knockdown and sh-Control HeLa cells were treated with Dox at the indicated time points or left untreated. Then, protein extracts were subjected to SDS-PAGE and immunoblotted with antibodies indicated.