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. 1998 Mar;116(3):1145–1150. doi: 10.1104/pp.116.3.1145

Figure 2.

Figure 2

Regulation of PG mRNA, enzyme activity, and protein accumulation by time of ethylene treatment. MG transgenic ACC synthase antisense tomato fruits were treated with 10 μL/L ethylene for the indicated times, and PG mRNA, enzyme activity, and protein levels were determined. A, mRNA-hybridization analysis. Each lane contained 3 μg of poly(A+) mRNA. B, PG enzyme assays were performed by incubating 10 mg of crude cell wall protein extracts with polygalacturonic acid, and PG activity was determined by a reducing sugar assay. C, Immunoblot of cell wall protein extracts. Each lane contained 10 mg of protein, and PG protein was detected with antibody by using a chemiluminescence kit. PG2 indicates the mobility of purified PG.