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. 2012 Nov 14;32(46):16503–16509. doi: 10.1523/JNEUROSCI.0209-12.2012

Figure 3.

Figure 3.

Detection of inclusions in Atg7 conditional knock-out mice. A, Inclusions (arrowheads) are present in the Atg7 conditional knock-out sections of 10-month-old mice. Ubiquitin (Ub)- and p62-positive inclusions are present in the cytoplasm of neuronal cells. Scale bar, 10 μm. B, Ubiquitin-containing inclusions (arrowheads) are present in juvenile (1-month-old) mice but are larger in size in adult (9-month-old) Atg7 flox/flox; DAT-Cre mice and Atg7 flox/flox; En-Cre mice. Scale bar, 20 μm. Cells were counterstained with Nissl. C, Western blot analysis of ubiquitin (Ub) in cortex (CTX) and midbrain/hindbrain (MHB) lysates obtained from Atg7 flox/flox control and Atg7 flox/flox; En-Cre mice. D, Quantitation of ubiquitin content (*p < 0.05). Atg7 flox/flox control mice (black) and Atg7 flox/flox; En-Cre mice (light gray).