Table I.
Sample | Salt Concentrationa | Apparent Molecular Massb |
---|---|---|
kD | ||
Chloroplast lysate | 150 mm NaCl | 420 |
40% (NH4)2SO4 supernatant | 150 mm KCl | 300 |
Purified complex | 150 mm NaCl or 150 mm KCl | 310 |
Purified complex | 500 mm (NH4)2SO4 | 340 |
Concentration and salt used during size-exclusion chromatography with the Sephadex G-200 matrix.
Estimated molecular masses are ± 12.5%, as described in Methods.