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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Dev Dyn. 2012 Oct 25;241(12):1911–1921. doi: 10.1002/dvdy.23876

Figure 1. Dynamic distribution of Cdc14A1 during the cell cycle at 8 hpf.

Figure 1

A: Structure of cdc14a1 gene and wild-type (WT) transcripts. Green bars indicate translation start codons and orange bars indicate in frame stop codons. B–D: Localization of Cdc14A1 in the ectoderm of live embryos at 8 hpf (B,C) and fixed embryos at 14 hpf (D). (B) The embryo was injected with synthetic cdc14a1_tv1-GFP (B, green in B”) and mcherry-centrin (B’, purple in B”) RNAs. Dashed lines delimit two cells in interphase. (C) Dividing cell proceeding through metaphase (00:00), anaphase (00:40–01:20) and telophase (2:00). Dashed lines delimit the mitotic cell. (D) The embryo was injected with synthetic cdc14a1_tv1-GFP RNA and stained using antibodies against GFP (D, green in D”), γ-tubulin and acetylated tubulin (D’, purple in D”). Yellow arrowheads point to the centrosomes. Red asterisk indicates the mitotic spindle. Blue arrows delimit the ends of the cilium. Scale bars represent 10 µm.