Figure 4.

Nontoxic doses of selenite enhance generation of reactive oxygen species in PC-3 cells sensitized with carmustine. PC-3 cells were pretreated with 10 or 20 μM carmustine for 30 minutes and then treated with the indicated concentration of selenite (1.5–5 μM) for 2 hours. Generation of reactive oxygen species was then measured as described in the Materials and methods section. The data are presented as the mean ± standard deviation (*P < 0.05; n = 3) and expressed as relative fluorescence (%). Data are normalized to the control. Comparisons shown: (a) significant compared with control; (b) significant compared with 1.5 μM selenite-treated cells; (c) significant compared with 2.5 μM selenite-treated cells.

Abbreviation: DCF, Dichlorofluorescein.