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Differentiation of long-term propagated neural tissue-spheres (NTS) derived from the embryonic porcine ventral mesencephalon (VM).
| Age (DIV) | Passage (P) | Protocol(s) | Duration (days) |
|---|---|---|---|
| 51 | (1) Mitogen-free | ||
| P2 | (2) a-FGF, forskolin, TPA, db-cAMP | 25 | |
| 170 | (1) Mitogen-free | ||
| P14 | (2) a-FGF, forskolin, TPA, db-cAMP | 10 | |
| 232 | (1) Mitogen-free | ||
| P18 | (2) FGF8 + sonic hedgehog | 10 |
