Table 2.
Summary of studies which have shown increased SKP2 expression in the presence of MYCN and/or MYCN amplification in neuroblastoma.
| Study | Tumors | Cell Lines | Methods | Evidence |
|---|---|---|---|---|
| Sugihara et al. (2006) | – | SHEP-MYCN1 | WB | E |
| Bell et al. (2007a) | – | IMR32, SKNBE2C, Tet21N2 | MYCN siRNA, microarray3, qRT-PCR | Si, E |
| Westermann et al. (2007) | 49/117/141/19 | |||
| Microarray/qRT-PCR/IHC tissue array/WB | – | Microarray, qRT-PCR, WB, IHC | T | |
| Chen et al. (2010) | – | Tet21N | Microarray | E |
| Muth et al. (2010) | 251 Microarray | SHEP, SHSY5Y, SKNSH, SKNBE2C, IMR32, IMR5-75, Kelly, Tet21N, IMR5-75-shMYCN4 | Microarray, SKP2 promoter assay, MYCN shRNA, qRT-PCR, WB | T, R, Sh, E |
1SHEP cells transfected with retroviral control vector or vector containing MYCN; 2GIMEN, NB69, LAN-6, NBL-S, PER-108, SMSKCNR, NBLW, CHLA-136, and NGP were analyzed for SKP2 protein expression using WB, however no correlation between SKP2 protein expression and MYCN protein expression or MYCN amplification was observed; 3Microarray analysis performed on IMR32 and SKNBE2C cells treated with scrambled or MYCN siRNA for 16 and 48 h; 4IMR5-75 cells with inducible stably transfected short hairpin RNAs against MYCN.
WB, Western blotting; IHC, immunohistochemistry; R, reporter gene assays; Si, differential expression following MYCN siRNA; Sh, differential expression following MYCN shRNA; E, differential expression/correlation in ectopic MYCN expression systems; T, correlation in tumors with MYCN amplification.