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. 2012 Jun 12;41(3):855–861. doi: 10.3892/ijo.2012.1516

Figure 6.

Figure 6

Aldehyde dehydrogenase 1A1 (ALDH1A1) knockdown enhances sensitivity of MIA PaCa-2/GR cells to GEM. (A) The results of MTT assay of MIA PaCa-2/P and MIA PaCa-2/GR cells, which were treated with gemcitabine (GEM) in a dose-dependent manner (0, 0.01, 0.1, 1 and/or 10 μM) for 72 h. Error bars represent the standard deviation. **P<0.01 represents significant difference between the MIA PaCa-2/GR cells plus GEM and MIA PaCa-2/P cells plus GEM groups. (B) Western blot analysis of exponentially growing MIA PaCa-2/P and MIA PaCa-2/GR cells was used to determine the expression level of ALDH1A1. (C) An MTT assay of MIA PaCa-2/GR cells transfected with ALDH1A1-siRNA or control-siRNA for 48 h, and then further treated with GEM in a dose-dependent manner (0, 0.01, 0.1, 1 and/or 10 μM) for 72 h. Error bars represent the standard deviation. **P<0.01 represents significant difference between the ALDH1A1-siRNA plus GEM and control-siRNA plus GEM groups.