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. Author manuscript; available in PMC: 2012 Nov 28.
Published in final edited form as: Exp Biol Med (Maywood). 2011 Mar 22;236(5):537–545. doi: 10.1258/ebm.2011.010367

Figure 2. Brachyury-inhibition reduces expression of mesenchymal markers, migration, and invasion of human lung carcinoma cells.

Figure 2

(A) Bright field images of H460 lung cancer cells stably transfected with a control (con.shRNA) or a Brachyury-specific shRNA (Br.shRNA) grown on plastic surface (top, X10 magnification) and immunofluorescent analysis of EMT markers (bottom, X20 magnification); merged images with DAPI stained nuclei are shown. (B) In vitro cell migration and ECM invasion assays. Error bar = SEM of triplicate measurements. * p<0.05, **p<0.001, for con.shRNA vs. Br.shRNA. Reproduced with permission from the American Society for Clinical Investigation; see reference 49.