Skip to main content
. 2012 Nov 7;32(45):16018–16030. doi: 10.1523/JNEUROSCI.1685-12.2012

Figure 5.

Figure 5.

Dnlg2 forms a biochemical complex with Dnrx. A, IPs from wild-type fly head lysates immunoblotted (IB) using anti-Dnlg2 antibodies show the presence of Dnlg2 (145 kDa, arrowhead). B, C, IP from wild-type fly head lysates using anti-Dnrx reveals the presence of Dnlg2 in the same complex (B, 145 kDa, arrowhead). The 70 kDa Dnlg2 does not associate with Dnrx. Only nonspecific background bands are observed in the wild-type and dnlg2 lysates near where the 70 kDa band is expected (C, arrowhead). Note that B and C are from the same protein blot probed separately. D, IPs from equal amounts of wild-type and dnlg2 mutant fly head lysates using anti-Dnlg2 show the presence of Dnlg2 (70 kDa) in wild-type but not in dnlg2 mutants. (Note that the break in between the lanes is due to removal of an empty lane). E, Dnrx protein levels are unaffected in dnlg2 mutants. F, IPs from equal amounts of wild-type and dnlg2 mutants using anti-Dnrx show the presence of Dnrx in both wild-type and dnlg2 mutants.