Figure 7.

Dnlg2 expression is required in presynaptic and postsynaptic areas for rescue of synaptic transmission defects in dnlg2 mutants. A–C, Quantification of electrophysiological analyses for dnlg2 and dnrx²⁷³ single and dnlg2;dnrx double mutants at muscle 6 of the third abdominal segment. dnlg2 and dnrx²⁷³ single and dnlg2;dnrx double mutants showed reduced EJP amplitudes (A) but normal mEJP amplitude (B). All the mutants have reduced quantal contents (C). Similarly, presynaptic (elav-Gal4) and postsynaptic (24B-Gal4 or C57-Gal4) expression of Dnlg2 in dnlg2^CL2 and dnlg2^KO70 mutants failed to restore EJP amplitudes (A) and quantal content (C) deficits. Ubiquitous expression of Dnlg2 using tub^P-Gal4 in both dnlg2^CL2 and dnlg2^KO70 mutants restores the EJP amplitude (A) to control levels. mEJP amplitudes (B) remain unchanged in dnlg2^CL2 mutants when Dnlg2 is expressed presynaptically and postsynaptically alone or in combination. However, an increase in mEJP amplitude is seen when Dnlg2 is expressed in muscles using C57-Gal4 in dnlg2^KO70 mutants, while no changes were observed when Dnlg2 is expressed using elav-Gal4 or tub^P-Gal4. ***p < 0.001; **p < 0.01; *p < 0.05 (Student's t test).