Table 1.
Peptide Backbone and Threonine Side Chain Vicinal Coupling Constants (Hz)a.
| Construct | PEP | A | B | C | D | E | F | G |
|---|---|---|---|---|---|---|---|---|
| 3JHN-Hα | ||||||||
| T2 | 7.8 | 8.8 | 7.9 | 8.0 | 8.9 | 8.6 | 7.8 | 8.9 |
| T3 | 8.0 | 8.3 | 8.9 | 8.1 | 9.1 | 8.3 | 8.9 | 9.5 |
| T4 | 7.4 | 6.8 | 7.6 | 8.3 | 7.2 | 8.0 | 8.5 | 8.5 |
| L6 | 6.6 | 6.3 | 6.3 | 5.9 | 6.1 | 5.7 | 5.5 | 5.5 |
| K7 | 7.2 | 7.3 | 7.3 | 6.7 | 7.2 | 6.7 | 7.3 | 7.0 |
| 3JHα-Hβ | ||||||||
| T2 | 4.5 | 2.2 | 5.0 | 4.4 | 2.6 | 2.6 | 5.1 | 2.4 |
| T3 | 4.4 | 3.7 | 2.0b | 5.1 | <2.0c | 4.2 | ND | ND |
| T4 | 6.2 | 5.9 | 5.7 | 2.2 | 6.1 | <2.0c | 2.2 | <2.0c |
a
glycosylated sites in bold, determined to better than +/− 0.2 Hz
b
coupling only partially resolved
c
coupling not resolved
d
ND not determined