Figure 2. Expression of the Lck-STIM1-C construct in HEK293 cells. (A) Representative live confocal image of cells expressing the Lck-STIM1-CT construct bearing a C-terminal eGFP-tag. (B) Mean (± SE) AA-activated ARC channel currents (black) and store-operated CRAC channel currents (gray) measured at -80 mV in siRNA-treated cells (siRNA), and in the same cells following expression of an siRNA-resistant wild-type STIM1 (+wt STIM1) or the siRNA-resistant Lck-STIM1-C construct (+Lck-STIM1-C). (C) Mean (± SE) I/V curves for the ARC channels (black) and CRAC channels (gray) in the siRNA-treated cells expressing the wild-type STIM1, and (D) the corresponding I/V curves for the ARC channels and CRAC channels in the siRNA-treated cells expressing the Lck-STIM1-C construct.