Figure 1. Overexpression of Stargazin C-terminal peptides (GFP-StgC14) blocks the S-SCAM-induced increase of surface AMPA receptor levels in hippocampal neurons. (A) Sequence alignment of the last 14 amino acids of various TARPs involved in the PDZ interaction. (B) Representative images of surface GluA2 staining in dendritic segments of neurons transfected with GFP alone (control), GFP-StgC14 alone, GFP + myc-S-SCAM, or GFP-StgC14 + myc-S-SCAM. (C) Quantification of the effect of Stg-C14 and S-SCAM on surface GluA2. Scale bar represents 5 µm. ***p < 0.001; n.s, not significant; n = 30 per condition.