Table 2.
Orthology analysis of selected proteins known to play a significant role in the immunity of C. elegans. The protein names for C. elegans are given in the leftmost column with protein descriptions given in the second column from the left. The number of proteins found in orthology clusters with the proteins in the leftmost column are labeled under each species examined. The orthology analysis was run using the species listed across the top and also included B. xylophilus and P. pacificus as non-parasitic nematodes as well as Nasonia vitripennis, the parasitoid wasp, as an arthropod outgroup (see Appendix 1 for details). All protein data was taken from whole genome releases except for L. oneistus, for which protein data from transcriptomics was used. All individual orthology results and the protein identifiers for the clustered orthologs can be found in Supplemental Table 1 (http://www.biolbull.org/content/supplemental). Those nematodes with limited or no free-living stages are indicated with grey stars (
) above their names.
| C. elegans protein | Protein description | C. elegans | L. oneistus | S. carpocapsae |
B. malayi
|
A. suum
|
T. spiralis
|
||
|---|---|---|---|---|---|---|---|---|---|
| TGF-beta | DBL-1 | TGF-beta ligand | 1 | 1 | 1 | 1 | 1 | 0 | |
| SMA-6 | Type I TGF-beta receptor | 1 | 1 | 1 | 2 | 1 | 1 | ||
| SMA-2 & SMA-3 | Smad protein | 3 | 2 | 3 | 3 | 3 | 2 | ||
| SMA-4 | Smad protein | 1 | 1 | 1 | 3 | 2 | 1 | ||
| Insulin/IGF-1 | GOA-1 | G protein alfa subunit | 1 | 1 | 1 | 0 | 1 | 1 | |
| DGK-1 | Diacylglycerol kinase beta | 1 | 2 | 1 | 4 | 1 | 1 | ||
| INS-7 | Insulin/IGF-1-like peptide | 8 | 0 | 0 | 0 | 0 | 0 | ||
| Epithelial cell | DAF-2 | Insulin/IGF-1 receptor | 1 | 1 | 3 | 4 | 2 | 2 | |
| AGE-1 | Phosphotidylinositol 3-kinase | 1 | 3 | 1 | 1 | 1 | 0 | ||
| AKT-1 | Rac Ser/Thr protein kinase | 1 | 3 | 0 | 0 | 0 | 0 | ||
| AKT-2 | Rac Ser/Thr protein kinase | 1 | 1 | 1 | 1 | 1 | |||
| SGK-1 | Serum/glucocorticoid regulated kinase 1 | 1 | 2 | 1 | 0 | 1 | 0 | ||
| DAF-16 | FOXO family transcription factor | 1 | 2 | 2 | 2 | 1 | 1 | ||
| p38 MAPK pathway | Epidermal immunity | RACK-1 | G protein beta subunit | 1 | 1 | 2 | 1 | 1 | 1 |
| PLC-3 | Phospholipase C gamma | 1 | 1 | 2 | 1 | 1 | 1 | ||
| PKC-3 | Protein kinase C iota type | 1 | 1 | 1 | 2 | 1 | 1 | ||
| GPA-12 | G protein alpha subunit | 1 | 0 | 1 | 1 | 1 | 1 | ||
| NIPI-3 | Tribbles homolog 1 (TRB-1) | 1 | 1 | 1 | 1 | 1 | 0 | ||
| Intestinal immunity | EGL-30† | G protein G(q) alpha subunit | 1 | 2 | 1 | 2 | 2 | 1 | |
| EGL-8† | Phospholipase C beta homolog | 1 | 0 | 2 | 2 | 1 | 2 | ||
| DKF-2 | Ser/Thr protein kinase D | 1 | 2 | 1 | 2 | 1 | 1 | ||
| RAB-1 | Ras-related GTPase Rab-1A | 1 | 5 | 1 | 1 | 1 | 2 | ||
| NSY-1 | ASK1 MAPKKK | 1 | 2* | 2 | 2 | 1 | 1 | ||
| SEK-1 | MKK3, MKK6, MAPKK | 1 | 4 | 1 | 1 | 1 | 1 | ||
| PMK-1 | p38 MAPK | 1 | 3 | 1 | 1 | 1 | 1 | ||
| Other immune effectors | SPP-10 | Saposin-like protein | 1 | 3 | 2 | 1 | 1 | 0 | |
| LYS-8 | Lysozyme | 5 | 1 | 2 | 1 | 0 | 0 | ||
| LYS-4,5,6, & 10 | Lysozyme | 4 | 5 | 2 | 0 | 1 | 0 | ||
| CLEC-48 & 50 | C type domain-containing proteins (CTLD) | 3 | 23 | 2 | 1 | 1 | 0 | ||
| CLEC-178 | CTLD | 1 | 3 | 0 | 0 | 1 | 0 | ||
| CLEC-56 | CTLD | 5 | 1 | 2 | 0 | 0 | 0 | ||
| CLEC-3,10, & 11 | CTLD | 43 | 3 | 0 | 0 | 0 | 0 | ||
| CLEC-150 | CTLD | 1 | 1 | 0 | 0 | 0 | 0 | ||
| FIP-1-like | FIDR protein | 1 | 1 | 0 | 0 | 0 | 0 |
EGL-30 and EGL-8 are known to be involved in both the Insulin/IGF-1 pathway as well as intestinal immunity in C. elegans.
No NSY ortholog was identified in L. oneistus; L. oneistus proteins identified in this cluster are orthologs of human TFG-beta activated kinase MAPKKK7.