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. 2012 Oct 8;11:123. doi: 10.1186/1475-2840-11-123

Table 1.

The sequences of forward (F) and reverse (R) primers and restriction enzymes for genotyping of the ten SNPs

SNP Primer sequence Restriction enzyme PCR product Allele
ABCA-1 V825I
F: 5′-GGTAGCCCACCACTCTCCCCTATAAAG-3′
TagI
525 bp
G
(rs2066715)
R: 5′-ATCAGCTGCCTGTCCTTGGACTA-3′
 
 
A
ACAT-1
F: 5′-TATATTAAGGGGATCAGAAGT-3′
RsaI
389 bp
A
(rs1044925)
R: 5′-CCACCTAAAAACATACTACC-3′
 
 
C
LDL-R AvaII
F: 5′-GTCATCTTCCTTGCTGCCTGTTTAG-3′
AvaII
228 bp
A-
 
R: 5′-GTTTCCACAAGGAGGTTTCAAGGTT-3′
 
 
A+
LIPC -250G>A
F: 5′-GGCAAGGGCATCTTTGCTTC-3′
DraI
411-bp
G
(rs2070895)
R: 5′-GGTCGATTTACAGAAGTGCTTC-3′
 
 
A
LIPG 584C>T
F: 5′-CATGAGCTGAGATTGTTGTCAGTGC-3′
NdeI
254 bp
C
(rs2000813)
R: 5′-CAGTCAACCACAACTACATTGGCGTCTTTCTCTCAT-3′
 
 
T
MTHFR 677C>T
F: 5′-CAAAGGCCACCCCGAAGC-3′
HinfI
254 bp
C
(rs1801133)
R: 5′-AGGACGGTGCGGTGAGAGTG-3′
 
 
T
MYLIP
F: 5′-ACAGAGCAAAAGACCCTGTCTC-3′
HaeIII
387 bp
G
(rs3757354)
R: 5′-AAAGAACTGTGTGTGGGAGGAT-3′
 
 
T
PCSK9 E670G
F: 5′-CACGGTTGTGTCCCAAATGG-3′
Eam 1104I
440 bp
A
(rs505151)
R: 5′-GAGAGGGACAAGTCGGAACC-3′
 
 
G
PPARD +294T>C
F: 5′-CATGGTATAGCACTGCAGGAA-3′
BslI
269 bp
T
(rs2016520)
R: 5′-CTTCCTCCTGTGGCTGCTC-3′
 
 
C
SCARB1
F: 5′-CCTTGTTTCTCTCCCATCCTCACTTCCTCGACGC-3′
HinI1
218 bp
C
(rs5888) R: 5′-CACCACCCCAGCCCACAGCAGC-3′     T