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Validation of a multiplex PCR. Lanes 1, 7: 100 bp DNA ladder, lane 2: negative control (no template), lane 3: C. obsoletus, lane 4: C. scoticus, lane 5: C. chiopterus, lane 6: C. dewulfi. The multiplex PCR mix contained obs-COI-fwd, sco-COI-fwd, chio-COI-fwd, dew-COI-fwd and PanCuli-COX1-727R primers.
