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Testing the multiplex PCR on field-collected crushed midges morphologically pre-identified to Obsoletus complex/C. dewulfi. Lane 1: 1 kb DNA ladder, lane 2: negative control (no template), lane 3: C. dewulfi, lanes 4, 5, 6, 10, 11, 14, 15: C. obsoletus, lanes 7, 8, 9, 13: C. scoticus, lane 12: C. chiopterus, lane 16: C. scoticus/C. chiopterus.
