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Exemplary flow cytometric analysis of an ischemic hemisphere at 24 h after MCAO. Vital brain cells can be clearly identified within the forward (FSC) / sideward (SSC) scatter plot. These cells can be further distinguished into a CD45-negative (i) and a CD45-positive fraction, the latter containing CD45low (ii) resting microglia and CD45high (iii) infiltrated leukocytes.
