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. 2012 Sep 20;5:513. doi: 10.1186/1756-0500-5-513

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Copyright ©2012 Dimauro et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Example western blots from three starting samples. In the top panel; (A) myoblast sample (20 μg protein was added to each lane) fractionated into nuclei, cytoplasmic and mitochondrial samples. Each fraction was run side-by-side on the same blot and then probed separately against each of the three primary antibodies, Histone-H3, GAPDH and CoxIV, to validate fraction purity. This was repeated for; (B) myotubes (20 μg protein per lane) and (C) AT tissue (15 μg protein per lane). In the bottom panel; marker protein fraction expression was measured as OD per resultant band area and was expressed in arbitrary units. The histogram data are representative of the mean (± SEM) of five separate experiments.