Table 1.
Fraction yields for myoblast, myotube and AT tissue
|
Sample |
Mean protein yield (μg) |
Total sample protein (mg) |
No. of replicates |
||
|---|---|---|---|---|---|
| Nuclei | Cyto | Mito | |||
|
Myoblast |
1179 ± 100 (150μL) |
496 ± 25 (100μL) |
97 ± 13 (50μL) |
1.8 ± 0.1 |
5 |
|
Myotube |
1624 ± 107 (150μL) |
547 ± 4 (100μL) |
197 ± 15 (50μL) |
2.4 ± 0.2 |
5 |
| AT tissue | 3294 ± 254 (300μL) | 1486 ± 225(300μL) | 444 ± 60 (100μL) | 5.2 ± 0.5 | 5 |
Typical protein yield (μg) obtained after sample fractionation using the indicated volume of lysis buffer for myoblasts, myotubes (both cultured in 75 cm2 flask, ≈2×106 cells) and AT muscle (all n = 5, mean ± SEM). The protein yields (determined by BCA assay as total volume of a fraction × protein concentration in the fraction) are representative and dependent upon the volume of buffer utilized to resuspend each fraction.