Table 1.

Primer sequences for MHC in the brown bear

Primer pair no.	Primer name	Primer sequence (5’-3’)	Gene	Amplicon size (bp)
1	1UNIex1L	TCCTsCTGCTrCTsTCGG	MHC class I, 1st exon	625
	1UNIex4R	GCCCAGCACCTCAGGGTG	MHC class I, 4th exon
2	2DRBUNIex1L	GAyryTGATGrTGCTGArC	MHC class II DRB, 1st exon	343
	2DRBUNIex3R	CCAGGAGGkTGTGrTGCTGCA	MHC class II DRB, 3rd exon
3	2DQAUNIex1L	CCwsTGGArGTGAAGAsAT	MHC class II DQA, 1st exon	255
	2DQAUNIex3R	AACACwGTsACCTCAGGAAC	MHC class II DQA, 3rd exon
4	2DQBUNIex1L	GGCTGAGGGCAGAGACTC	MHC class II DQB, 1st exon	282
	2DQBUNIex3R	TGGAyGGGrAGATrGTCACTGT	MHC class II DQB, 3rd exon
5	URS_1_F	GCTCsCACTCCCTGAGGTAT	MHC class I,	228
	URS_1_R	CCwCGCTCTGGTTGTAGTA	2nd exon
6	URS_DRB_F_3	TTCACCAACGGsACGGAGCGG	MHC class II DRB,	192
	URS_DRB_R	CTTGTCGCyGCACCrkGAAGCT	2nd exon
7	URS_DQA_F	CTGACCATGTTGCTTACTACGG	MHC class II DQA,	202
	URS_DQA_R	CATTGGTAGCAGCGrTATAGTTGGA	2nd exon
8	URS_DQB_F	AGGATTTCGTGCTCCAGyTTAAG	MHC class II DQB,	224
	URS_DQB_R	CTCGCCGCTGCrGGATGArsCTG	2nd exon
9	URS_1_F	CGTATCGCCTCCCTCGCGCCATCAG	MHC class I, 2nd exon	228
		xxxxxxGCTCSCACTCCCTGAGGTAT
	URS_1_R	CTATGCGCCTTGCCAGCCCGCTCAG
		xxxxxxCCwCGCTCTGGTTGTAGTA
10	URS_DRB_F_3	CGTATCGCCTCCCTCGCGCCATCAG	MHC class II DRB, 2nd exon	192
		xxxxxxTTCACCAACGGsACGGAGCGG
	URS_DRB_R	CTATGCGCCTTGCCAGCCCGCTCAG
		xxxxxxCTTGTCGCyGCACCrkGAAGCT

xxxxxx indicates the location of a 6-bp tag in the fusion primer sequences.