Figure 2. Negative pressure hyperpolarizes Na_V1.5 voltage-dependence of activation (V_1/2a) and inactivation (V_1/2i). Shown here are voltage-dependent current vs. voltage (IV) of activation (squares) and inactivation (circles) from inside-out patches at resting pressure (0 mmHg, black) and with applied pressure (-30 mmHg, red). Data were fit to two-state Boltzmann functions (solid lines) and shows a hyperpolarizing shift in the half-points of voltage dependence of activation (ΔV_1/2a = -10 mV) and inactivation (ΔV_1/2i = -12 mV) (n = 5, p < 0.01).