Figure 3. Lidocaine inhibits pressure-induced hyperpolarization of Na_V1.5 voltage-dependence of activation (V_1/2a) and but not inactivation (V_1/2i). Shown here are voltage-dependent current vs. voltage (IV) of activation (squares) and inactivation (circles) from five inside-out patches at resting pressure (0 mmHg, black) and with applied pressure (-30 mmHg, red). Data was fit to two-state Boltzmann functions (solid lines) and shows a a -4 mV shift in the half-point of voltage dependence of activation (ΔV_1/2a) (n = 7, p < 0.05), and a -10 mV shift in the half-point of voltage dependence of inactivation (n = 7, p < 0.05).