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. 2012 Nov 28;7(11):e50432. doi: 10.1371/journal.pone.0050432

Figure 4. EMT marker and ECM protein analysis on undifferentiated hPSC, PTK7−, PTK7+ and DE populations.

Figure 4

Feeder-free H9 and XFiPSC2 (differentiated for 2 days) were sorted into PTK7+ and PTK7− populations. Expression level was determined by microarray analysis as described in Materials and Methods. All samples were normalized to PTK7− from the same sort. Shown are the averages of normalized readings for PTK7+ and PTK7−, with standard error across two experiments. The relative expression of all samples was analyzed for (A) EMT markers, (B) ECM proteins and (C) genes that are upregulated in PTK7+ samples.