Figure 4. cfos silencing with siRNAs inhibits the cAMP-mediated expression of c-jun and CD44.

(A) cfos siRNA silencing efficiency assessed by RT-PCR analysis from NB4-LR1 cells treated with 8-CPT-cAMP (150 µM). Specific pool siRNAs reduce cfos mRNA levels by at least 80%. (B–D) Silencing of cfos results in inhibition of c-jun (B), CD44 standard form (CD44s) (C) and CD44 splice variant (CD44v) (D) expression, as assessed by RT-PCR analysis using specific primers.