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. 2012 Nov 28;7(11):e50407. doi: 10.1371/journal.pone.0050407

Figure 4. Glutamine reduces the JNK phosphorylation and PARP-1 proteolysis induced by TNBS-colitis.

Figure 4

(A–C) Protein from colonic extracts was separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, followed by immunoblotting for JNK, phospho-JNK (cytosolic extracts) and PARP-1 (nuclear extracts). Phospho-JNK and PARP-1 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished phospho-JNK and PARP-1 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin (cytosolic extracts) and lamin B (nuclear extracts) bands. (A) Representative Western-blot photographs for JNK, phospho-JNK, β-actin, PARP-1 and lamin-B. (B) Densitometric quantification of phospho-JNK. (C) Densitometric quantification of PARP-1. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. #P<0.05 compared with TNBS group.