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Comparative qPCR. Murine ERα, BRCA1, ErbB2, and PR mRNA expression were compared in MC7-L1 ER+ and ERαKD cell lines. RNA extraction was performed in triplicate for each cell line, and each qPCR reaction (each sample, each gene) was repeated thrice. Data were expressed relative to one of the three MC7-L1 samples for each gene.
