Figure 3.

In-vivo study: Immunohistochemical analysis of 8-isoprostane in lung tissue. Immunohistochemical analysis of 8-isoprostane in lung tissue was performed in formalin-fixed, paraffin-embedded mouse lungs from: (A) untreated mice, (B) non sensitized mice exposed to UfCP (UfCP group), (C) sensitized and challenged mice exposed to filtered air (S/OVA group), and (D) sensitized mice exposed to UfCP prior to OVA challenge (S/UfCP/OVA group), retrieved 7 days after UfCP exposure/OVA challenge. Arrowheads indicate bronchial and bronchiolar epithelial cells; arrows indicate inflammatory cells including alveolar macrophages. Scale bar, 50 μm.