Figure 4.

In-vivo study: Immunohistochemical analysis of PGE₂ in lung tissue. Immunohistochemical analysis of PGE₂ in lung tissue was performed in formalin-fixed, paraffin-embedded mouse lungs from: (A) untreated mice, (B) non sensitized mice exposed to UfCP (UfCP group), (C) sensitized and challenged mice exposed to filtered air (S/OVA group), and (D) sensitized mice exposed to UfCP prior to OVA challenge (S/UfCP/OVA group) retrieved 7 days after UfCP exposure/OVA challenge. (E) untreated mice, (F) sensitized and challenged mice exposed to filtered air (S/OVA group), and (G) sensitized mice exposed to UfCP prior to OVA challenge (S/UfCP/OVA group) retrieved 1 day after UfCP exposure/OVA challenge. Arrowheads indicate bronchial and bronchiolar epithelial cells; arrows indicate inflammatory cells including alveolar macrophages. Scale bar, 50 μm.