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. 2012 Aug 2;12:129. doi: 10.1186/1471-2229-12-129

Figure 2.

Figure 2

Molecular characterisation of transgenic PG1as apple lines. Western analysis of protein extracts from fruit of transgenic PG1as lines and ‘Royal Gala’ (RG) control after SDS-PAGE (8 μg protein per lane) at harvest (A), and after ripening for 16 weeks at 5°C (B), using a polyclonal antibody raised to recombinant apple PG1. mRNA abundance of PG1(C), and a ripening-related apple β-GALACTOSIDASE gene (D)[38], at harvest (H) and after ripening for 16 weeks at 5°C (16 w). mRNA abundance of the PG1as-41 sample at harvest was set as 1 (n = 4 ± Standard Error).