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. 2012 Aug 2;12:129. doi: 10.1186/1471-2229-12-129

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Copyright ©2012 Atkinson et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Polyuronide content and size distribution of water-soluble and CDTA-soluble polyuronides from the cell walls of transgenic PG1as and ‘Royal Gala’ (RG) control apple fruit after ripening for 16 weeks at 5°C.A) Polyuronide content, expressed as mg of uronic acid per g fresh weight of tissue, of water-soluble extracts (white bars) and CDTA-soluble extracts (grey bars) (n = 3 ± Standard Deviation). B) Size distribution of water-soluble polyuronides, and (C) CDTA-soluble polyuronides after elution on Sepharose CL-2B (profiles are typical examples from two extractions). Arrows indicate elution of dextran molecular weight markers (kDa).