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. 2012 Dec;26(12):4903–4913. doi: 10.1096/fj.12-213934

Figure 4.

Figure 4.

PLCβ1 expression does not affect siRNA down-regulation of several proteins. A) Left panels: identical study as described in Fig. 3, except using siRNAs to cyclophilin and Hsp90; n = 4. B) Reduction in PLCβ1 levels promotes siRNA(GAPDH) activity in uninduced HEK-β1 and HeLa cells. Western blot band intensities of GAPDH and Hsp90 of cells with various siRNA treatment; n = 2. C) Control study showing that PLCβ1 overexpression reverses siRNA(GAPDH) in HeLa cells. D) Top panel: level of Ago-2 pulled down by PLCβ1 in tetracycline vs. untreated cells; n = 2. Bottom panel: Western blot bands corresponding to PLCβ1 that were pulled down by streptavidin beads from HEK293 cells induced for PLCβ1 expression, and treated with either siRNA(GAPDH), siRNA(Hsp90) or no siRNA. Error bars = sd.