Figure 5.

PLCβ1 increases cell survival in siRNA(LDH)-treated cells. A) Left panel: HEK293-TAP-PLCβ1 cells were treated with siRNA(LDH) and/or tetracycline (tet) to induce PLCβ1 expression and were counted 24 and 48 h after treatment; n = 4. Error bars = sd. Right panel: Western blot analysis showing the presence of LDH in cells that survived siRNA (LDH) treatment and the absence of LDH in nonsurviving cells. B) Linkage of siRNA(LDH) or siRNA (GAPDH) to H2B expression. Western blot and Coomassie-stained gel showing that down-regulation of LDH and GAPDH eliminates H2B expression in the detached cells as compared to attached (i.e., healthy) cells that express H2B; n=3. Protein loading was assessed by Ponceau staining (see Supplemental Fig. S6).