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. 2012 Nov 28;7(11):e49450. doi: 10.1371/journal.pone.0049450

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© 2012 Kashiwakura et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PCR analysis of genomic DNA of 134-fetus was shown. (A) Two or three independent PCR reactions were carried out for detection of recombination in F8 of 134-fetus. (B) Southern blotting with a 5′ exon 14 probe (on Sac I− or Sac I + Stu I-digested DNA) and with a 3′ exon 22 probe (on Sph I− or Xba I-digested DNA) showed correct targeting of the F8 in 134-fetus.