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. 2012 Nov 28;7(11):e48819. doi: 10.1371/journal.pone.0048819

Figure 2. Digital gene expression profiling (DGEP).

Figure 2

A, Outline of experimental process. B, Principle and procedure in detail; Beads of Oligo (dT) are used to enrich mRNA in the total RNA, and then are transferred into double-stranded cDNA through reverse transcription. Four base recognition enzyme NlaIII is used to digest this cDNA, and Illumina adapter 1 is linked. Mmel is used to digest at 17 bp downstream of CATG site; Illumina adapter 2 is linked at 3′ end. Primer GX1 and Primer GX2 are added for PCR. Then, regain 85 bp strips through 6% TBE PAGE. The DNA was purified and followed by Solexa sequencing.