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. 2012 Nov 28;7(11):e49146. doi: 10.1371/journal.pone.0049146

Figure 9. In vivo engraftment of HA nanostructured scaffolds (van Gieson staining, 20×).

Figure 9

(A) DPSCs from the younger group (16–25). (B) DPSCs from the senior group (>66). *, HA granules; black arrows, extracellular matrix. (C) Real-time PCR. Time course of osteogenic (osteopontin, osteonectin, osteocalcin, collagen type I, Runx2) and vasculogenic (CD31, vWF, VEGF) mRNA expression analyzed by semi-quantitative real-time PCR of HA nanostructured scaffolds in vivo embedded with DPSCs after 21 days. The results for each experiment are from quadruplicate experiments. Values are expressed as the mean ± SD. T tests were used to determine significant differences (p<0.05). * p<0,05; * * p<0,01; * * * p<0,001.