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. 2012 Oct 31;12(5):207–212. doi: 10.4110/in.2012.12.5.207

Figure 1.

Figure 1

TIM-3 mRNA expression was increased in HMC-1 cells by stimulation with TGF-β1 but not by stimulation with IFNs, IL-10, and TNF-α. HMC-1 cells were treated for the indicated time with IFN-α (100 U/ml), IFN-λ (100 ng/ml), TGF-β1 (2 ng/ml), IL-10 (10 ng/ml), and TNF-α (10 ng/ml), respectively and then TIM-3 transcript levels were analyzed by real-time RT-PCR. TIM-3 transcript levels were normalized to GAPDH transcript levels. Relative Tim-3 mRNA levels to control (Con, without cytokine treatment) were presented. *p<0.005.