Figure 2.

Basal TIM-3 promoter activity in HEK293 and HMC-1 cells. (A) Luciferase reporter vectors containing various length of TIM-3 promoter region. (B) HEK293 (5×10⁵) or (C) HMC-1 (1×10⁶) cells were transfected with the indicated luciferase reporter vector together with pEGFP-N1 vector (1/10 of total plasmid) and 48 h later luciferase activity was measured and normalized to the frequencies of GFP-expressing cells. Relative luciferase activity to control (pGL-Basic) was presented. The data are the mean±standard deviation of 5 experiments. ^*p<0.01.