Figure 4. Binding of ATX to T cells.

(A) Flow cytometry analysis of b-ATX staining of mouse T cells in the presence (red) or absence of Mn⁺² (blue trace). CTRL here and in panels B–D denotes staining with b-ATX omitted. (B) b-ATX staining (with Mn⁺² present) of T cells in the presence or absence of PS/2, an anti α4 integrin antibody. (C) b-ATX staining (with Mn⁺² present) of T cells from wild-type mice, β2-deficient T cells (ltgβ2 null mice), and β3-deficient T cells (ltgβ3 null mice) (D) b-ATX staining (with Mn⁺² present) of T cells in the presence or absence of a 10-fold excess of T210A. (E) Fluorescent imaging of TK1 cells stained with b-ATX (green) and CD44 (red). Top row shows rounded cells bound to substratum and bottom row shows polarized cells (induced with 1 μM LPA). Scale bar denotes 10 μM. Data are representative of at least 4 independent experiments